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Updated: Jul 16, 2026

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
Published on: April 30, 2011
NMR study of 100 kDa HCV IRES RNA using segmental isotope labeling
Insil Kim1, Peter J Lukavsky, Joseph D Puglisi
1Department of Structural Biology, School of Medicine, Stanford University, California 94305-5126, USA.
Abstract:
RNA NMR is hindered by the large size of most biological RNAs. We present here a simple method for segmental isotopic labeling of an RNA fragment within the context of a larger RNA. The methodology uses transcription and ribozyme cleavage to prepare appropriate ends for RNA ligase catalyzed ligation. We demonstrate that a 64 nucleotide domain of the Hepatitis C virus internal ribosome entry site (IRES) RNA adopts an independently folded domain within the context of the intact, 100 kDa IRES.

