Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A paramagnetic MRI-CEST agent responsive to lactate concentration.

Silvio Aime1, Daniela Delli Castelli, Franco Fedeli

  • 1Dipartimento di Chimica IFM, V. P. Giuria 7, I-10125 Torino, Italy. silvio.aime@unito.it

Journal of the American Chemical Society
|August 9, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Peptide-Guided Photodynamic Therapy via Integrin αvβ6 in Pancreatic Cancer.

International journal of molecular sciences·2026
Same author

Microbubble-free mechanical sonoporation for MR-guided drug delivery in solid tumours: a proof-of-concept study.

Drug delivery and translational research·2026
Same author

Solution Structures and Dynamics of Diamagnetic Lu- and Y-DOTAM/Fluoride Ternary Complexes: The Role of <sup>19</sup>F-CEST in the Detection of the Complex Fluoride Speciation.

Inorganic chemistry·2026
Same author

Calcifying nested stromal-epithelial tumor of the liver: Report of two cases revealing novel WT1 mutation and distinct epigenetic features.

Virchows Archiv : an international journal of pathology·2025
Same author

In situ sonoporation to enhance the tumour uptake of silicon phthalocyanine and improve PDT effectiveness in a triple negative breast cancer murine model.

Journal of photochemistry and photobiology. B, Biology·2025
Same author

Preclinical evaluation of [<sup>68</sup>Ga]Ga-AAZTA-FAPI-46: a novel PET tracer for targeting fibroblast activation protein (FAP).

EJNMMI radiopharmacy and chemistry·2025

Researchers developed a novel Yb(III) complex for MRI-CEST imaging. This paramagnetic agent detects L-lactate concentration by altering its chemical exchange saturation transfer properties.

Area of Science:

  • Inorganic Chemistry
  • Biomedical Imaging
  • Nuclear Magnetic Resonance (NMR) Spectroscopy

Background:

  • Paramagnetic Yb(III) complexes are explored for Magnetic Resonance Imaging (MRI) contrast agents.
  • Chemical Exchange Saturation Transfer (CEST) is a sensitive MRI technique.
  • Developing targeted contrast agents for specific biomarkers like L-lactate is crucial.

Purpose of the Study:

  • To investigate a Yb(III) complex, [Yb(MBDO3AM)](3+), as a potential MRI-CEST contrast agent.
  • To assess the complex's responsiveness to L-lactate concentration.
  • To evaluate the feasibility of using NMR time scale exchange and proton resonance separation for independent CEST assessment.

Main Methods:

  • Synthesis and characterization of the paramagnetic Yb(III) complex [Yb(MBDO3AM)](3+).

Related Experiment Videos

  • Investigation of the complex's binding with L-lactate to form [Yb(MBDO3AM)L-lactate](2+).
  • Nuclear Magnetic Resonance (NMR) spectroscopy to study exchange dynamics and proton resonance frequencies.
  • Assessment of Chemical Exchange Saturation Transfer (CEST) properties of both free and bound complex forms.

    • Main Results:

    • The Yb(III) complex binds L-lactate quantitatively.
    • Slow exchange on the NMR time scale and significant proton resonance separation (>10 ppm) were observed.
    • Independent assessment of CEST properties for free and L-lactate-bound complex forms was achieved.
    • The resulting saturation transfer to bulk water signal demonstrated dependence on L-lactate concentration.

    Conclusions:

    • The investigated Yb(III) complex functions as a responsive MRI-CEST contrast agent for L-lactate.
    • The distinct CEST properties of the free and bound states enable L-lactate quantification.
    • This paramagnetic agent holds promise for targeted MRI-based biomarker detection.