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Direct wiring of cytochrome c's heme unit to an electrode: electrochemical studies
Jianjun Wei1, Haiying Liu, Allison R Dick
1Contribution from the Chemistry Department, University of Pittsburgh, Pennsylvania 15260, USA.
Abstract:
A novel strategy for the immobilization of cytochrome c on the surface of chemically modified electrodes is demonstrated and used to investigate the protein's electron-transfer kinetics. Mixed monolayer films of alkanethiols and omega-terminated alkanethiols (terminated with pyridine, imidazole, or nitrile groups that are able to ligate with the heme) are used to adsorb cytochrome c to the surface of gold electrodes. The use of mixed films, as opposed to pure films, allows the concentration of adsorbed cytochrome to remain dilute and ensures a higher degree of homogeneity in their environment. The adsorbed protein is studied using electrochemical methods and scanning tunneling microscopy.
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