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Updated: Sep 30, 2026
Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts
Published on: July 27, 2015
Peroxidatic activity in heart effluent: a biochemical parameter for the assessment of experimental
1"Carol Davila" University of Medicine and Pharmacy, Faculty of Medicine, Department of Cell Biology and Histology, 8 Eroilor Sanitari Blvd., 76241 Bucharest, Romania. mleabu@univermed-cdgm.ro
Abstract:
Peroxidatic activity in heart effluent was defined as a new biochemical parameter for the experimental study of myocardial ischemia. The peroxidatic reaction was determined by dot blot analysis with 3,3'-diaminobenzidine as hydrogen donor. After ischemia, the level of peroxidatic activity in heart effluent was 2-3 times higher than before. The effects in experimental modulation of ischemia, such as nicorandil or aprikalim protection, and the reversibility of protection by glibenclamide, could accurately be noted using the level of peroxidatic activity in heart effluent as a biochemical parameter. The results were in good agreement with those obtained for other enzymes used as biochemical parameters in experimental heart ischemia-reperfusion studies.
