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cDNA RDA Cloning of the Gene Specifically Expressed in Murine Nasopharynx

Jian-Hua Ling1, Kai-Tai Yao

  • 1Cancer Research Institute, Human Medical University, Changsha 410078, China. klcarcrnch@public.cs.hn.cn

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|August 9, 2002
PubMed

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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