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Cloning and Sequencing of the Gene Encoding NS Protein of Wheat Rosette Stunt Virus
Jia-Li Shen1, Yong-Jun Ye, Zu-Xun Gong
1Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. gongzx@sunm.shcnc.ac.cn
Abstract:
The cDNA fragment located downstream of the WRSV N protein gene was obtained from the cDNA library of WRSV by hybridization with a 24 nucleotides sequence fragment of the 3' end of mRNA of the N protein. The cDNA fragment was sequenced and on ORF encoding a 40 kD protein was found. The gene was expressed in E. coli DE3 and was identified by Western blot experiment as the NS gene of WRSV.
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