Studies on Light Harvesting Complexes of Photosystem II in a Chlorophyll-deficient Barley Mutant NYB

As compared with wild type barley, the LHCII content of NYB barley mutant reduced markedly and its polypeptide components also changed: the contents of 24 kD, 27 kD and 30 kD polypeptides reduced and 26 kD polypeptide was missing. Northern blot analysis with specific cab gene probe showed that there was no difference in the LHCII mRNA level between the wild and mutant barley, indicating the decreased LHCII level in mutant barley was not due to a reduced transcription or accumulation of its mRNA. The rudimentary development state of thylakoids in NYB barley may be correlated with the loss of 26 kD polypeptide.