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Cloning and Characterization of Mouse Glial Cell Line-derived Neurotrophic Growth Factor (GDNF)
Fu-Jun Wang1, Jian Zhao, Too H p
1Department of Applied Biology, East China University of Science and Technology, Shanghai 200237, China. wfj@npc.haplink.com.cn, wfj@public1.sta.net.cn
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|August 9, 2002
Abstract:
We have cloned two unique forms of glial cell line-derived neurotrophic growth factor transcripts (GDNF-alpha and GDNF-beta) from adult mouse brain. The GDNF-beta form is similar to GDNF-alpha expect for a 78 bp deletion in the ORF. The deduced amino acid sequences of the mouse GDNF-alpha are highly homologous to those of rat and human GDNF. Reverse-transcription PCR from various mouse tissues showed extensive expression of both forms of GDNF. GDNF-alpha is coded by at least two distinct exons. The 3apos-UTR is entirely encoded in a single exon.