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Rapid release of Ole e 1 from olive pollen using different solvents
J Carnés1, E Fernández-Caldas, L Boluda
1C.B.F. LETI, S.A., Research & Development Department, Tres Cantos, Madrid, Spain.
Allergy
|August 10, 2002
Summary
Deionized water rapidly releases Ole e 1, a major olive allergen, from pollen. Other solvents like phosphate-buffered saline and saline affect allergen solubility and release kinetics, influencing antigenic profiles.
Area of Science:
- Allergen research
- Pollen analysis
- Biochemistry
Background:
- Solubility is a critical property of allergenic molecules.
- Ole e 1 is a primary allergen found in Olea europaea (olive) pollen.
Purpose of the Study:
- To investigate the solubility of Ole e 1 using various solvents.
- To understand how different solvents affect Ole e 1 release and antigenic profiles.
Main Methods:
- Olive pollen extraction using deionized water, phosphate buffer (PBS), and normal saline (NaCl).
- Analysis of eluted fractions using SDS-PAGE and immunoblots.
- Quantification of Ole e 1 content and calculation of 50% inhibition protein amounts.
- Skin prick testing on patients with deionized water fractions.
Main Results:
- Deionized water yielded four elution profiles, while PBS and saline produced three each.
- Ole e 1 release was fastest with deionized water (256 µg/ml in 7 min).
- PBS and saline showed delayed Ole e 1 release, with peak concentrations of 162 µg/ml and 203 µg/ml, respectively.
Conclusions:
- The choice of extracting solvent significantly impacts the antigenic profile of olive pollen extracts.
- Deionized water facilitates rapid Ole e 1 release, while other solvents affect solubility and kinetics.
- These findings suggest methods to manipulate Ole e 1 content in O. europaea extracts.