Related Experiment Video
Updated: Sep 30, 2026

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
Published on: October 31, 2014
Palindromic unit-independent transposition of IS1397 in Yersinia pestis
Caroline Wilde1, Sophie Bachellier, Maurice Hofnung
1Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS URA 1444, Laboratoire des Yersinia, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
Abstract:
Palindromic units (PUs) are intergenic repeated sequences scattered over the chromosomes of Escherichia coli and several other enterobacteria. In the latter, IS1397, an E. coli insertion sequence specific to PUs, transposes into PUs with sequences close to the E. coli consensus. Reasons for this insertion specificity can relate to either a direct recognition of the target (by its sequence or its structure) by the transposase or an interaction between a specific host protein and the PU target DNA sequence. In this study, we show that for Yersinia pestis, a species deprived of PUs, IS1397 can transpose onto its chromosome, with transpositional hot spots. Our results are in favor of a direct recognition of target DNA by IS1397 transposase.
More Related Videos
Related Concept Videos
Plague
Transposons
Translesion DNA Polymerases
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Overview of Transposition and Recombination
Transduction
Coordination of Gene Expression Processes in Bacteria

