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Related Experiment Video

Updated: Jun 25, 2026

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

The von Hippel-Lindau tumor suppressor stabilizes novel plant homeodomain protein Jade-1.

Mina I Zhou¹, Hongmei Wang, Jonathan J Ross

¹Department of Medicine, Section of Nephrology, Boston University School of Medicine and Boston Medical Center, Boston, Massachusetts 02118, USA.

The Journal of Biological Chemistry

|August 10, 2002

Summary

Researchers identified Jade-1 as a novel protein partner of the von Hippel-Lindau (VHL) tumor suppressor. VHL protein stabilizes Jade-1, revealing a new mechanism in renal cancer suppression.

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Last Updated: Jun 25, 2026

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Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

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Published on: October 14, 2022

Area of Science:

Molecular Biology
Oncology
Genetics

Background:

The von Hippel-Lindau (VHL) gene is implicated in most adult renal cancers.
The precise mechanism of VHL protein's tumor suppressor function in the kidney is not fully understood.

Purpose of the Study:

To identify novel, low-occupancy VHL protein partners relevant to renal cancer.
To elucidate the functional relationship between VHL and Jade-1 in renal tumorigenesis.

Main Methods:

Yeast two-hybrid screening of a human kidney library against VHL p30.
Characterization of Jade-1 protein expression, localization, and interaction with VHL.
Assessment of VHL's effect on Jade-1 protein abundance and half-life in renal cancer cells.

Main Results:

Identified Jade-1, a novel 64-kDa protein highly expressed in the kidney, as a strong VHL interactor.
Jade-1 is localized in the cytoplasm and nucleus, partly colocalizing with VHL, and is abundant in renal cancer precursor cells.
VHL reintroduction into renal cancer cells significantly increased Jade-1 abundance (up to 10-fold) and half-life (up to 3-fold).

Conclusions:

Direct protein stabilization of Jade-1 by VHL is a newly identified VHL function.
Jade-1 represents a novel candidate regulatory factor in VHL-mediated renal tumor suppression.
Understanding the VHL-Jade-1 interaction may offer new therapeutic strategies for renal cancer.