Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

N-phenylethyl amitriptyline in rat sciatic nerve blockade.

Peter Gerner1, Mustafa Mujtaba, Mohammed Khan

  • 1Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA. gerner@zeus.bwh.harvard.edu

Anesthesiology
|August 10, 2002
PubMed
Summary

N-phenylethyl amitriptyline shows potent Na(+) channel blockade for chronic pain relief, offering significantly longer nerve block duration than lidocaine but with a narrow therapeutic range. Further studies are needed for neuropathic pain applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute Interstitial Nephritis Is a Potential Adverse Effect in Inflammatory Bowel Disease Patients Treated With Vedolizumab: A Case Report and Review of the Literature.

Kidney medicine·2026
Same author

Immune and non-immune hydrops fetalis in a Saudi tertiary center: etiologies, antenatal predictors, perinatal outcomes, and one-year survival in a seven-year cohort.

Frontiers in pediatrics·2026
Same author

Evaluating a targeted educational intervention to promote high-value, cost-conscious care among medical students.

Medical education online·2026
Same author

Outcomes Following Regenerative Peripheral Nerve Interface Surgery and Associations With Preoperative Mental Health: A Retrospective Analysis of Upper-Extremity Neuromas.

Journal of hand surgery global online·2026
Same author

TRAF6 coiled-coil domain mediates its trimerization.

bioRxiv : the preprint server for biology·2026
Same author

Urinary pesticide profiles and liver disease risk in Thailand: a machine-learning risk-prediction model.

medRxiv : the preprint server for health sciences·2026

Area of Science:

  • Neuroscience
  • Pharmacology
  • Anesthesiology

Background:

  • Amitriptyline, an antidepressant, treats chronic pain via Na(+) channel blockade.
  • Neuropathic pain involves high-frequency ectopic discharge.
  • Modifying amitriptyline with a phenylethyl group may enhance its properties.

Purpose of the Study:

  • To assess the electrophysiologic properties of N-phenylethyl amitriptyline.
  • To evaluate its therapeutic range and toxicity in vivo.

Main Methods:

  • Whole-cell patch clamp technique on cultured neuronal GH(3) cells.
  • Rat sciatic nerve model for evaluating nerve block duration and neurotoxicity.

Main Results:

  • N-phenylethyl amitriptyline demonstrated significantly greater in vitro Na(+) channel block (approx. 90%) than amitriptyline (approx. 15%).

Related Experiment Videos

  • It retained amitriptyline's use-dependent blockade property.
  • In vivo, it provided a 1,932 min block duration, far exceeding lidocaine (72 min), but showed neurotoxicity at higher concentrations.

    • Conclusions:

    • N-phenylethyl amitriptyline is a potent local anesthetic with extended block duration.
    • It exhibits a narrow therapeutic range.
    • Potential utility in neuropathic pain warrants further investigation in animal models.