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[Dual-color FISH study on myelodysplastic syndrome with 1;7 translocation]
Yongmei Shen1, Yongquan Xue, Jianyong Li
1First Affiliated Hospital of Suzhou University, Jiangsu Institute of Hematology, Suzhou, Jiangsu, 215006 P.R.China. uujihsmc@public1.sz.js.cn
Objective:
To study the myelodysplastic syndrome(MDS) with 1;7 translocation in five cases and to determine further the constitution and origin of centromere of the derivative chromosome resulting from 1;7 translocation.
Methods:
Bone marrow chromosome preparation of five cases was made using direct method or short- term culture. Karyotypic analysis was carried out by R-banding technique. Dual-color fluorescence in situ hybridization(FISH) using Spectrum Red and Spectrum Green directly labeled chromosome 1-specific a-satellite DNA probe(red) and chromosome 7-specific a-satellite DNA probe(green) was performed in three patients of them.
Results:
All of the five cases had 1;7 translocation. The centromere of the derivative chromosome 7p/1q was constituted with red and green signals in three of them.
Conclusion:
The result of dual-color FISH confirms that the centromere of the derivative chromosome resulting from 1;7 translocation originated from both centromeres of chromosome 1 and chromosome 7.