Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse brain protein composition during postnatal development: an electrophoretic analysis.

P T Kelly1, M W Luttges

  • 1Department of Psychobiology, University of California, Irvine, Irvine, CA 92717, USA.

Journal of Neurochemistry
|November 1, 1976
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Breast cancer risk analysis: A genetic epidemiology service for families.

Journal of genetic counseling·2013
Same author

Another perspective on genetic risk assessment for cancer.

Journal of genetic counseling·2013
Same author

Ethical sourcing of dental instruments and materials.

British dental journal·2010
Same author

Effect of simulated commercial flight on oxygenation in patients with interstitial lung disease and chronic obstructive pulmonary disease.

Thorax·2004
Same author

Will cancer risk assessment and counseling services survive genetic testing?

Acta oncologica (Stockholm, Sweden)·1999
Same author

Nitric oxide acts as a postsynaptic signaling molecule in calcium/calmodulin-induced synaptic potentiation in hippocampal CA1 pyramidal neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience·1999
Same journal

Splice Type-Specific Effects of Gαo Subunits on Cerebellar Anatomy and Synapse Formation.

Journal of neurochemistry·2026
Same journal

Neurobiological and Behavioral Consequences of Prenatal Methadone or Buprenorphine Exposure: Insight From Animal Models.

Journal of neurochemistry·2026
Same journal

Parkinson's Disease Cell Transplantation Therapy: A New Dawn With Pluripotent Stem Cell-Based Therapy.

Journal of neurochemistry·2026
Same journal

Neural Organoid Models as a Platform for Studying Disease Mechanisms in Amyotrophic Lateral Sclerosis.

Journal of neurochemistry·2026
Same journal

LRRK2 Mutation Alters Dopamine D2 Receptor Localization in Induced Pluripotent Stem Cells-Derived Astrocytes From Parkinson's Disease Patients: Implications for Neuronal Damage.

Journal of neurochemistry·2026
Same journal

GM<sub>1</sub>-Oligosaccharide Rescues Rotenone-Impaired Neuronal Polarization Through RhoA/ROCK Modulation and Mitochondrial Protection.

Journal of neurochemistry·2026
See all related articles

Mouse brain protein concentrations change during development, particularly in myelin. Researchers used gel electrophoresis to analyze these protein shifts, ensuring accurate results by addressing potential contamination issues.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Molecular Biology

Background:

  • Understanding brain development requires characterizing changes in protein expression during postnatal maturation.
  • Subcellular fractionation and electrophoresis are key techniques for analyzing protein composition.

Purpose of the Study:

  • To investigate changes in mouse brain protein concentrations during postnatal development.
  • To identify specific protein alterations in different subcellular fractions, especially myelin.

Main Methods:

  • Subcellular fractionation to isolate cellular components (nuclei, mitochondria, microsomes, cytosol, myelin).
  • Sodium dodecyl sulfate gel electrophoresis (SDS-PAGE) to separate and quantify proteins.
  • Use of exponential gradient gels for enhanced resolution of low molecular weight proteins.

Related Experiment Videos

  • Analysis of histone concentrations in isolated nuclei, with and without Triton X-100 treatment.

    • Main Results:

    • Significant changes in protein concentrations were observed across various subcellular fractions during postnatal maturation.
    • Myelin membrane fractions exhibited the most pronounced maturational changes in protein content.
    • Exponential gradient gels improved the resolution and detection of low molecular weight myelin proteins.
    • Histone concentrations in purified nuclei remained stable during maturation, and Triton X-100 treatment mitigated contamination effects.

    Conclusions:

    • Postnatal brain maturation involves dynamic alterations in protein composition across subcellular compartments.
    • Myelin proteins undergo significant changes, highlighting their role in brain development and function.
    • Methodological considerations, such as preventing nuclear fraction contamination, are crucial for accurate protein analysis.