Donor cells for nuclear cloning: many are called, but few are chosen

The few viable clones obtained at the end of a typical cloning experiment are genetic copies of the donor cell genome of a non-reproductive (somatic) or embryonic cell used for nuclear transfer. Nuclear totipotency has to be reestablished by erasing epigenetic constraints imposed on the donor genome during differentiation in a process which involves active chromatin remodeling. Various donor cell types and cell cycle combinations have proven to be capable of generating cloned offspring. However, an ideal nuclear donor may have not yet been found. This review summarizes current theoretical aspects of donor cell selection. It focuses on the impact of genetic and epigenetic differences between donor cell types on successful mammalian cloning.