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Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Specific interaction of p53 with target binding sites is determined by DNA conformation and is regulated by the

Thomas Göhler1, Maurice Reimann, Dimitry Cherny

  • 1Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Martinistrasse 52, Hamburg D-20251, Germany.

The Journal of Biological Chemistry
|August 13, 2002
PubMed

Insights

DNA topology influences tumor suppressor p53

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • p53's transcriptional activation relies on sequence-specific DNA binding.
  • Regulation of p53 DNA binding is multifaceted and occurs at multiple levels.

Purpose of the Study:

  • To investigate the role of DNA topology in regulating p53's selective DNA binding.
  • To elucidate how DNA conformation affects p53-target site interactions.

Main Methods:

  • Analysis of p53 binding to DNA with varying topological properties.
  • Characterization of the C-terminal domain's role in DNA conformation-dependent binding.

Main Results:

  • Specific binding of wild-type p53 is significantly enhanced by non-linear stem-loop DNA conformations.
  • The C-terminal domain is crucial for p53 binding to non-linear DNA but inhibits binding to linear DNA.

Conclusions:

  • DNA topology is a critical regulator of p53's selective and specific binding to target sites.
  • The conformation of p53 cognate sites may determine selective p53 binding to various promoters.

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