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Protein array technology. Potential use in medical diagnostics.
K Büssow1, Z Konthur, A Lueking
1Max Planck Institute of Molecular Genetics, Berlin, Germany. buessow@molgen.mpg.de
American Journal of Pharmacogenomics : Genomics-Related Research in Drug Development and Clinical Practice
|August 14, 2002
Summary
Functional genomics research uses whole-genome expression profiling to identify gene functions. This study links gene catalogs to functional catalogs using high-throughput screening of cDNA libraries and microarrays.
Area of Science:
- Genomics
- Molecular Biology
- Biotechnology
Background:
- The human genome sequence is known, yet the functions of most genes remain uncharacterized.
- Functional genomic studies are crucial for understanding gene roles, particularly in disease and development.
- High-throughput gene activity monitoring is essential for analyzing complex expression patterns.
Purpose of the Study:
- To develop and validate a method for linking gene sequences to their functional protein products.
- To establish a high-throughput system for functional genomic analysis using cDNA libraries and microarrays.
- To bridge the gap between DNA sequencing data and functional gene catalogs.
Main Methods:
- Utilized high-density filter membranes and microarrays for gene expression profiling.
- Employed cDNA libraries for high-throughput protein expression and antibody screening.
- Integrated DNA hybridization, sequencing, and functional assays for clone analysis.
Main Results:
- Demonstrated the utility of cDNA libraries for both DNA hybridization and protein-based screening.
- Established a direct link between identified gene clones and their corresponding protein products.
- Showcased microarrays as a platform to move from individual clones to specific genes and proteins.
Conclusions:
- Developed a unified approach for functional genomics, connecting gene catalogs with functional catalogs.
- Enabled comprehensive database integration of the entire process from DNA sequencing to functional assays.
- Advanced high-throughput methods for assigning functions to newly discovered genes.