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Preparation and Biological Activity of [B(1)Ala, B(2)Ala, B(3)Lys]-Insulin
Ying Ye1, Ming-Hua Xu, Xin-Tang Zhang
1Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. zhusq@sunm.shcnc.ac.cn
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|August 14, 2002
Abstract:
This paper describes the preparation and biological activity of an insulin analogue in which the B1-3 sequence (Phe-Val-Asn) of insulin is substituted by Ala-Ala-Lys. [B(1)Ala, B(2)Ala, B(3)Lys]-Insulin retains full in vivo activity and receptor binding activity as insulin, but its lipogenesis activity and immunoactivity are 70 % and 0.88 % of those of insulin respectively. The possible contribution of the N-terminus of B-chain of insulin to the structure and function of insulin is discussed.