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Analysis of C-terminal Amidating Structure and Determination of Amidating Enzyme Activity by Using Capillary
Zhi-Hong Jiang1, Hong-Min Tu, Yu-Hong Yang
1Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
Abstract:
Based on the difference in charge and hydrophobicity between amidating moiety and the carboxyl group in a given buffer solution, a new method to analyze the C-terminal amidating structure and the amidating enzyme activity by using capillary electrophoresis was established.