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Related Experiment Video

Updated: Jul 8, 2026

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope (AFM-SECM)
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Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope (AFM-SECM)

Published on: February 10, 2021

Combined scanning electrochemical/optical microscopy with shear force and current feedback.

Youngmi Lee1, Zhifeng Ding, Allen J Bard

  • 1Department of Chemistry and Biochemistry, University of Texas at Austin, 78712, USA.

Analytical Chemistry
|August 15, 2002
PubMed
Summary

A novel scanning electrochemical/optical microscopy (SECM/OM) technique integrates topographic, electrochemical, and optical imaging. This method enables simultaneous multi-modal analysis of diverse samples, including living cells.

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Area of Science:

  • Analytical Chemistry
  • Microscopy Techniques
  • Nanotechnology

Background:

  • Limitations in current microscopy techniques for simultaneous multi-modal analysis.
  • Need for advanced imaging methods to probe complex biological and material surfaces.
  • Development of integrated probe tips for combined functionalities.

Purpose of the Study:

  • To develop and demonstrate a simultaneous scanning electrochemical/optical microscopy (SECM/OM) technique.
  • To enable combined topographic, electrochemical, and optical imaging with a single probe.
  • To validate the technique on various sample types, including living cells.

Main Methods:

  • Design of a specialized probe tip integrating an optical fiber core, gold ring electrode, and insulating sheath.
  • Utilizing a quartz crystal tuning fork for shear force sensing to maintain constant tip-substrate distance.
  • Employing SECM tip current as an alternative feedback signal for soft samples.

Main Results:

  • Successful simultaneous acquisition of topographic, electrochemical, and optical images of an interdigitated array electrode.
  • Demonstration of simultaneous electrochemical and optical imaging of soft samples like polycarbonate filters and living diatoms.
  • Validation of constant-force and constant-current modes for different sample types.

Conclusions:

  • The developed SECM/OM technique offers unprecedented simultaneous multi-modal imaging capabilities.
  • This technique is versatile, applicable to both hard electrodes and soft biological samples.
  • The constant-current mode shows significant potential for mapping biochemical activity in living cells.