Image reconstruction through turbid media under a transmission-mode microscope
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Updated: Jun 22, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 9, 2014
1Swinburne University of Technology, Centre for Micro-Photonics, School of Biophysical Sciences and Electrical Engineering, P.O. Box 218 Hawthorn 3122, Victoria, Australia. xgan@swin.edu.au
Abstract:
In this paper, image enhancement and reconstruction through a turbid medium by utilizing polarization gating and mathematical image reconstruction methods in a microscopic imaging system are investigated. A Monte Carlo simulation model based on Mie theory and the concept of the effective point spread function (EPSF) is adopted to study image formation under a transmission-mode microscope. The results show that polarization gating methods, and particularly the differential polarization gating method, can be efficient in suppressing highly scattered light, which leads to a significant enhancement of image quality. An image reconstruction method based on the concept of the EPSF is demonstrated to be efficient in further improving image quality.
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