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Related Experiment Videos

Enhanced transdermal transport by electroporation using anionic lipids.

Arindam Sen1, Yali Zhao, Lei Zhang

  • 1Department of Molecular and Cellular Biophysics, Roswell Park Cancer Institute, Buffalo, NY 14263-0001, USA. arindam.sen@roswellpark.org

Journal of Controlled Release : Official Journal of the Controlled Release Society
|August 15, 2002
PubMed
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Anionic lipids combined with electroporation significantly enhance transdermal drug delivery for small molecules. This novel approach shows promise for improving drug transport across the skin barrier.

Area of Science:

  • Biomedical Engineering
  • Pharmaceutics
  • Materials Science

Background:

  • Transdermal drug delivery offers local or systemic treatment advantages.
  • Active transdermal methods like electroporation and liposomal delivery are evolving.
  • Combining lipids and electroporation may yield synergistic effects for enhanced drug transport.

Purpose of the Study:

  • To investigate the synergistic effect of anionic lipids and electroporation for transdermal molecule transport.
  • To evaluate the influence of molecule size and charge on lipid-enhanced electroporation.
  • To explore a novel transdermal delivery system using anionic lipids.

Main Methods:

  • Utilized heat-stripped porcine epidermis for in vitro transdermal transport measurements.

Related Experiment Videos

  • Prepared anionic lipid vesicles using 1,2-dioleoyl-3-phosphatidylglycerol (DOPG) and 1,2-dioleoyl-3-phosphatidylcholine (DOPC) at a 1:1 mole ratio.
  • Assessed transdermal transport of small molecules (Methylene Blue, Protoporphyrin IX, Dimethyl-Protoporphyrin IX) and large molecules (FITC-dextrans) with and without lipids under electroporation.

    • Main Results:

    • Electroporation-induced transdermal transport increased when anionic lipids (DOPG:DOPC) were mixed with target molecules.
    • Transport enhancement was molecule-dependent, observed for small molecules (<1 kDa) and 4 kDa FITC-dextran.
    • No significant enhancement was seen for larger dextrans (>10 kDa) or with neutral/cationic lipids.

    Conclusions:

    • Anionic lipids, specifically DOPG:DOPC vesicles, enhance electroporation-mediated transdermal delivery of small molecules.
    • The efficacy of this lipid-enhanced electroporation is dependent on the size and charge of the transported molecule.
    • This study presents a promising strategy for optimizing transdermal drug delivery systems.