Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optimization of bacteriorhodopsin for bioelectronic devices.

Kevin J Wise1, Nathan B Gillespie, Jeffrey A Stuart

  • 1Department of Chemistry, University of Connecticut, Storrs, CT 06269, USA.

Trends in Biotechnology
|August 15, 2002
PubMed
Summary

Genetic engineering significantly enhances bacteriorhodopsin (BR) for photonic devices. This bioelectronic optimization achieved a 700-fold improvement in volumetric data storage, showcasing the potential of directed evolution.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physiological media and oxygen levels modulate cancer hallmarks in cultured breast cancer cells.

The FEBS journal·2026
Same author

Applying the Lessons of Physiological Cell Culture to Human Embryo Culture for In Vitro Fertilization.

Biomolecules·2026
Same author

Polyunsaturated fatty acid metabolism in the retinal pigment epithelium and its association with outer retinal disease.

Mammalian genome : official journal of the International Mammalian Genome Society·2026
Same author

The scientific legacy of Martin Karplus from the perspective of his collaborators.

Biophysical journal·2026
Same author

Identifying genetic determinants of outer retinal function in mice using a large-scale gene-targeted screen.

PLoS genetics·2025
Same author

A biometric survey of known and prospective murine models of posterior microphthalmia-nanophthalmia.

Experimental eye research·2025

Area of Science:

  • Biophysics
  • Materials Science
  • Biotechnology

Background:

  • Bacteriorhodopsin (BR), a proton pump from Halobacterium salinarum, exhibits remarkable photochemical efficiency and stability.
  • Organic photonic materials have not yet matched BR's native quantum efficiency or cyclicity.
  • Commercial applications of BR are limited by its inherent efficiency.

Purpose of the Study:

  • To investigate the potential of genetic engineering to improve bacteriorhodopsin's device capabilities.
  • To explore bioelectronic optimization for enhanced protein performance in photonic applications.

Main Methods:

  • Utilized genetic engineering techniques to modify bacteriorhodopsin.
  • Employed semi-random mutagenesis and directed evolution strategies.

Related Experiment Videos

  • Assessed performance improvements in volumetric data storage applications.

    • Main Results:

    • Achieved a 700-fold improvement in volumetric data storage capacity using engineered BR.
    • Demonstrated significant enhancements in protein device capabilities through genetic modification.
    • Validated the effectiveness of bioelectronic optimization for BR.

    Conclusions:

    • Genetic engineering offers substantial improvements for protein-based photonic devices.
    • Directed evolution and mutagenesis are key strategies for future bioelectronic optimization.
    • Engineered bacteriorhodopsin shows promise for advanced data storage and bioelectronic applications.