Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Study on the interactions between CuL(2) and Morin with DNA.

Yumin Song1, Jingwan Kang, Zhihua Wang

  • 1Department of Chemistry, Northwest Normal University, Lanzhou 730070, China.

Journal of Inorganic Biochemistry
|August 15, 2002
PubMed
Summary

Morin and its copper complex bind to calf thymus DNA through distinct mechanisms. The copper complex intercalates into DNA, while Morin binds non-intercalatingly, altering DNA

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chirality-Matching in Au-S Oriented Assembly Induces Highly Selective Recognition of Glucose Enantiomers.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Sub-Nanometer PtSn Interlayer Tuning Ligand and Strain Effects Boosts Oxygen Reduction Electrocatalysis.

Angewandte Chemie (International ed. in English)·2026
Same author

Boosting Interface Dynamics via Axial Coordination Engineering to Enhance Photoelectrochemical Water Splitting Performance.

ACS applied materials & interfaces·2026
Same author

Molecularly Imprinted Aggregation-Induced Electrochemiluminescence Interface Based on Quinoline-Malononitrile Nanomaterials for Specific and Ultrasensitive Recognition of Zearalenone.

Analytical chemistry·2026
Same author

Insight Into the Porphyrin-Modulated Interfacial Charge Transfer Kinetics Behavior of Molybdenum Disulfide Nanostructures by Scanning Photoelectrochemical Microscopy.

ChemSusChem·2026
Same author

Excellent Electrochemiluminescence Performance of Carbazolyl Benzene via Substituent Optimization: A Structure-Performance Relationship Study.

Analytical chemistry·2026

Area of Science:

  • Biophysical Chemistry
  • Molecular Interactions
  • DNA Binding Studies

Background:

  • Flavonoids and their metal complexes are investigated for biological activities.
  • Understanding DNA-binding interactions is crucial for drug development and molecular biology.

Purpose of the Study:

  • To investigate the interaction of Morin and its copper complex (CuL2) with calf thymus DNA.
  • To elucidate the binding modes and spectral characteristics of Morin-DNA and CuL2-DNA interactions.

Main Methods:

  • Spectroscopic studies (absorption and fluorescence) to monitor interactions.
  • Viscometric studies to assess changes in DNA structure.
  • Ethidium bromide (EB) displacement assay to probe intercalation.

Related Experiment Videos

Main Results:

  • CuL2 exhibits distinct spectral changes with DNA compared to Morin.
  • CuL2 enhances DNA fluorescence, while Morin quenches it; CuL2 addition quenches DNA-EB fluorescence more significantly than Morin.
  • Viscosity and UV-Vis spectra indicate CuL2 binds to DNA via intercalation, while Morin binds non-intercalatingly, with CuL2 decreasing DNA's denaturation temperature.

Conclusions:

  • Morin and its copper complex interact differently with calf thymus DNA.
  • The copper complex (CuL2) primarily binds to DNA through intercalation.
  • Morin binds to DNA via a non-intercalating mode.