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Flow cytometry assay for intracellular rabies virus detection
Juliano Bordignon1, Silvia Cardoba Pires Ferreira, Graciane Maria Medeiros Caporale
1Laboratory of Immunology, Department of Microbiology and Parasitology at Federal University of Santa Catarina, SC Florianópolis, 88040-900 Brazil.
Journal of Virological Methods
|August 15, 2002
Summary
Flow cytometry effectively monitors rabies virus infection in cell lines. This method detects intracellular rabies virus early, providing a reliable way to study viral kinetics.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Flow cytometry is a recognized method for microbiological diagnosis.
- Previous studies have explored flow cytometry for detecting various infections.
Purpose of the Study:
- To evaluate flow cytometry for monitoring intracellular rabies virus infection in cell lines.
- To determine the kinetics of rabies virus infection using flow cytometry.
Main Methods:
- BHK-21 and C6 cell lines were infected with Pasteur Virus strain (PV) or wild rabies virus strain (WRS).
- Cells were fixed, permeabilized, and stained with rabies virus-specific fluorescent antibody conjugates.
- Flow cytometry (Becton Dickinson FACSCalibur) was used to quantify infected cells over time.
Main Results:
- Significant rabies virus infection levels (4.7-7.1%) were detected as early as 12 hours post-inoculation (P<0.01).
- Maximum infection levels reached 88% in C6 cells at 48 hours for both virus strains.
- Flow cytometry enabled precise determination of infection kinetics.
Conclusions:
- Flow cytometry is a sensitive and efficient method for monitoring intracellular rabies virus infection.
- This technique allows for quantitative analysis of viral infection dynamics in cell cultures.
- The study highlights the advantages of flow cytometry for studying virus-cell interactions.