Development of a new method to characterize (SMBV) antigen formulations using surface plasmon resonance technology

Area of Science:

• Biotechnology
• Nanotechnology
• Drug Delivery Systems

Background:

• Supra Molecular Biovectors (SMBV) are nanoparticles featuring a polysaccharidic core and lipid bilayer.
• SMBV are engineered for nasal administration in drug delivery and vaccine applications.
• Evaluating the association kinetics and stability of active principles (AP) or antigens (Ag) with SMBV is crucial for formulation development.

Purpose of the Study:

• To evaluate the association rate and stability of active principles (AP) or antigens (Ag) with Supra Molecular Biovectors (SMBV).
• To utilize plasmon resonance technology for rapid screening of SMBV formulations.
• To identify the optimal SMBV and formulation process for specific antigens.

Main Methods:

• Utilized Surface Plasmon Resonance (SPR) technology with a BIAcore X system and a Hydrophobic Protein Array (HPA) sensor chip.
• Injected solutions of AP, Ag, and/or adjuvant molecules over an SMBV-saturated HPA sensor chip surface.
• Employed a minimal quantity of material for rapid assessment of complex SMBV formulations.

Main Results:

• Demonstrated the capability of SPR to assess the association rate and stability of AP/Ag with SMBV.
• Confirmed SPR as a rapid screening technique for evaluating complex SMBV formulations.
• Showcased the efficiency of SPR in selecting optimal SMBV and formulation processes for specific antigens.

Conclusions:

• Plasmon resonance technology provides a rapid and efficient method for evaluating SMBV-based drug and vaccine formulations.
• This technique enables quick selection of the best SMBV and formulation strategies for antigen delivery.
• SPR significantly accelerates the development process for nasal delivery systems using SMBV.