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Structure of yeast RNA polymerase II in solution: implications for enzyme regulation and interaction with promoter
John L Craighead1, Wei-hau Chang, Francisco J Asturias
1Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Abstract:
An 18 A resolution structure of the 12-subunit yeast RNA polymerase II (RNAPII) calculated from electron microscope images of single particles preserved in amorphous ice reveals the conformation of the enzyme in solution. The Rpb4/Rpb7 polymerase subunit complex was localized and found to be ideally positioned to determine the path of the nascent RNA transcript. The RNAPII structure suggests a revised mode of interaction with promoter DNA and demonstrates that regulation of RNAPII must involve structural changes that render the enzyme competent for initiation.
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