Isoimmune anti-A blood group reagents in pigs

Immunization or reimmunization of A-negative pigs with red blood cells (RBC) from A-positive donors yielded anti-A antibodies reacting in high titres with phenotype A(Ac) RBC and, in some cases, in low dilutions, with phenotype Aw(Ap) RBC also. An attempt to raise the anti-A level by immunization with saliva which contained A substance was likewise successful. Repeated immunization of A-negative recipients with the RBC of A-positive donors (compatible in all other factors), with the aid of adjuvant, is recommended as the best way of obtaining Aw typing reagents.