Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Genetic Engineering?00:49

What is Genetic Engineering?

79.9K
Overview
79.9K
What is the Immune System?01:38

What is the Immune System?

127.2K
Overview
127.2K
Genetics of Speciation02:16

Genetics of Speciation

21.0K
Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
21.0K
What is Population Genetics?01:25

What is Population Genetics?

64.5K
A population is composed of members of the same species that simultaneously live and interact in the same area. When individuals in a population breed, they pass down their genes to their offspring. Many of these genes are polymorphic, meaning that they occur in multiple variants. Such variations of a gene are referred to as alleles. The collective set of all the alleles within a population is known as the gene pool.
64.5K
Humoral Immune Responses01:36

Humoral Immune Responses

83.5K
Overview
83.5K
Mitochondrial Precursor Proteins01:39

Mitochondrial Precursor Proteins

3.6K
Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70  chaperones are targetted to TOM20-TOM22 receptor complexes.
Most of the mitochondrial...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SedNet: A physics-informed operator-learning framework for rapid sedimentation velocity analytical ultracentrifugation analysis.

International journal of pharmaceutics·2026
Same author

Rendering compact formation and trajectory tracking for cyber unmanned ground vehicles.

Fundamental research·2026
Same author

Drum Filling of Thin-Film Freeze-Dried Monoclonal Antibody Powder in UniDose Powder Nasal Spray System.

Pharmaceutical research·2025
Same author

Development of an inhalable dry powder of mycobacteriophage D29 using thin-film freeze-drying.

International journal of pharmaceutics·2025
Same author

IgM has a better relative distribution in inflammation sites and tumor tissues than IgG.

Journal of nanobiotechnology·2025
Same author

Heat transfer characteristics of sublimation from frozen thin films.

International journal of pharmaceutics·2025
Same journal

Design, Synthesis and Characterization of Anti-CTLA4 Peptide Conjugated Gemcitabine Prodrug Nanoparticles for Targeted Lung Cancer Chemoimmunotherapy.

Pharmaceutical research·2026
Same journal

Leveraging Carnitine-functionalized Lipid Nanocarrier based Targeted Delivery of A1874 PROTAC for Glioblastoma.

Pharmaceutical research·2026
Same journal

Impact of Febrile State on Vancomycin Clearance in Pediatric Patients: Insights From Population Pharmacokinetic Modeling.

Pharmaceutical research·2026
Same journal

Sustained Intra-Articular Delivery of Triple Therapeutics Using a Phase-Transition Phospholipid-Based Gel for Effective Treatment of Gouty Arthritis.

Pharmaceutical research·2026
Same journal

Spray Dried Lysozyme Microspheres: Morphological Evolution and Enzymatic Activity Retention.

Pharmaceutical research·2026
Same journal

Colloidal Stability of Amorphous Nanoparticles in Solution: Impact of Stabilizer.

Pharmaceutical research·2026
See all related articles

Related Experiment Video

Updated: Jan 25, 2026

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies
15:00

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies

Published on: May 11, 2019

6.1K

Genetic immunization using nanoparticles engineered from microemulsion precursors.

Zhengrong Cui1, Russell J Mumper

  • 1Division of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington 40536-0082, USA.

Pharmaceutical Research
|August 16, 2002
PubMed
Summary
This summary is machine-generated.

Engineered plasmid DNA (pDNA)-coated nanoparticles enhance genetic immunization. This novel delivery system significantly boosts immune responses, including IgG titer and T-helper cell cytokine release, compared to traditional pDNA methods.

More Related Videos

Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning
07:37

Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning

Published on: May 10, 2019

8.7K
Reverse Microemulsion-mediated Synthesis of Monometallic and Bimetallic Early Transition Metal Carbide and Nitride Nanoparticles
07:47

Reverse Microemulsion-mediated Synthesis of Monometallic and Bimetallic Early Transition Metal Carbide and Nitride Nanoparticles

Published on: November 27, 2015

11.3K

Related Experiment Videos

Last Updated: Jan 25, 2026

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies
15:00

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies

Published on: May 11, 2019

6.1K
Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning
07:37

Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning

Published on: May 10, 2019

8.7K
Reverse Microemulsion-mediated Synthesis of Monometallic and Bimetallic Early Transition Metal Carbide and Nitride Nanoparticles
07:47

Reverse Microemulsion-mediated Synthesis of Monometallic and Bimetallic Early Transition Metal Carbide and Nitride Nanoparticles

Published on: November 27, 2015

11.3K

Area of Science:

  • Nanotechnology
  • Immunology
  • Biomaterials

Background:

  • Plasmid DNA (pDNA) genetic immunization elicits immune responses.
  • Current delivery systems for pDNA lack versatility and cell-targeting capabilities.
  • Novel delivery systems are needed to improve genetic immunization efficacy.

Purpose of the Study:

  • To develop and characterize cationic nanoparticles coated with pDNA for enhanced genetic immunization.
  • To investigate the potential of incorporating endosomolytic lipids and dendritic cell-targeting ligands to improve transfection and immune responses.

Main Methods:

  • Engineered cationic nanoparticles using microemulsion precursors (emulsifying wax and CTAB).
  • Coated nanoparticles with pDNA, incorporating endosomolytic lipid and/or mannan ligand.
  • Evaluated in vitro cell transfection and in vivo immune responses in Balb/C mice after subcutaneous injection.

Main Results:

  • Rapid engineering of stable, spherical cationic nanoparticles (approx. 100 nm).
  • pDNA-coated nanoparticles, especially with both lipid and ligand, significantly enhanced IgG titer (over 16-fold) and Th1-type cytokine release (up to 300% increase).
  • Demonstrated superior performance compared to "naked" pDNA.

Conclusions:

  • A novel method for engineering pDNA-coated nanoparticles was successfully developed.
  • These nanoparticles enhance both in vitro cell transfection and in vivo immune responses.
  • The findings suggest a promising new platform for genetic immunization strategies.