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Related Experiment Videos

Cardiac tissue engineering.

Thomas Eschenhagen1, Michael Didié, Jürgen Heubach

  • 1Institute of Experimental and Clinical Pharmacology and Toxicology, Department of Clinical Pharmacology, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany. thomas.eschenhagen@pharmakologie.uni-erlangen.de

Transplant Immunology
|August 16, 2002
PubMed
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Engineered heart tissues (EHTs) created from neonatal rat cardiac myocytes show functional and structural properties similar to native myocardium. These EHTs survived and vascularized after implantation, offering a potential new approach for cardiac tissue replacement.

Area of Science:

  • Regenerative Medicine
  • Cardiovascular Biology
  • Biomaterials Engineering

Background:

  • Myocardial infarction causes irreversible cardiac myocyte loss.
  • Cell transplantation strategies aim to replace lost cardiac tissue.
  • An alternative approach involves creating functional cardiac tissue grafts in vitro.

Purpose of the Study:

  • To develop and characterize three-dimensional engineered heart tissues (EHTs) for potential cardiac repair.
  • To evaluate the functional and morphological properties of in vitro-generated cardiac tissue.
  • To assess the viability and integration of EHTs following implantation.

Main Methods:

  • Neonatal rat cardiac myocytes were used to generate ring-shaped, three-dimensional EHTs.
  • Isometric force measurements were performed on electrically stimulated EHTs.

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  • Morphological and ultrastructural analyses were conducted using light and electron microscopy.
  • EHTs were implanted into the peritoneum of Fischer 344 rats to assess survival and vascularization.

    • Main Results:

    • Electrically stimulated EHTs demonstrated physiological responses including Frank-Starling behavior and inotropic/lusitropic effects.
    • EHTs exhibited functional characteristics of native myocardium with low passive tension and high active tension.
    • Morphological analysis revealed a well-organized, interconnected network of cardiac myocytes with developed subcellular structures.
    • Implanted EHTs survived for at least 14 days, maintained differentiated myocytes, and showed significant vascularization.

    Conclusions:

    • Engineered heart tissues (EHTs) possess functional and morphological properties comparable to intact ventricular myocardium.
    • EHTs represent a promising biomaterial for novel tissue replacement strategies in cardiac repair.
    • Successful in vivo survival and vascularization support the potential of EHTs for therapeutic applications.