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Published on: November 29, 2006
Cellobiose dehydrogenase production by Sclerotium species pathogenic to plants
1Division of Biochemical Engineering, Institute of Food Technology, University of Agricultural Sciences Vienna (Universität für Bodenkultur, BOKU), Muthgasse 18, A-1190 Wien, Austria.
Aims:
Evaluation of various Sclerotium spp. as producers of the biotechnologically attractive enzyme cellobiose dehydrogenase.
Methods And Results:
All isolates of S. coffeicola, S. delphinii and S. rolfsii grown in shaken flasks on a cellulose-based medium produced appreciable amounts of the extracellular enzyme cellobiose dehydrogenase.
Conclusions:
Cellobiose dehydrogenase seems to play an important role in phytopathogenic Sclerotium spp.; a possible function could be either in the degradation of rigid lignocellulose or as a protective mechanism against toxic quinones.
Significance And Impact Of The Study:
S. coffeicola and S. delphinii were identified as potent, not-yet-described producers of cellobiose dehydrogenase (CDH). The high levels of intact CDH produced by the different Sclerotium species should make them attractive producers for further studies and applications.
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