Related Experiment Videos
[Effect of exogenous hyaluronan on wound healing]
Da-lie Liu1, Xi-jun Li, Yang Zhang
1Department of Plastic Surgery, Shenyang Army General Hospital, Shenyang, Liaoning, P. R. China 110016.
Objective:
To investigate the mechanism of impeded wound healing by exogenous hyaluronan (HA).
Methods:
Wound healing models were established on 18 adult rabbit ears, which were randomly divided into 3 groups, the 2% HA treated-group (group A), the 1% HA treated-group(group B), and the PBS control group (group C). The process of wound healing was observed morphologically and histologically. The expression of alpha-smooth muscle actin in fibroblast was measured by immunohistochemical method.
Results:
1. The mean values of wound healing time of groups A, B and C were (11.7 +/- 0.6), (11.3 +/- 0.6), and (10.8 +/- 1.0) days respectively. Wound contraction was greater in group C than in group A and group B. 2. Compared with PBS controls, the collagen fibril was slender and arrayed regularly in HA treated wound. 3. The expression of alpha-smooth muscle actin was greater in group C than in groups A and B.
Conclusion:
It is one of reasons of impeded wound healing that exogenous HA inhibits the expression of alpha-smooth muscle protein and wound contraction. There exists dose-dependent effect.