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[Experimental study on bacteriostasis of chitosan and sodium hyaluronate]
Zhao-hui Tang1, Chun-lin Hou, Qing-quan Chen
1Department of Orthopedic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, P. R. China 200003.
Objective:
To compare the effect and coverage of bacteriostasis of chitosan and sodium hyaluronate.
Methods:
Each of the five bacteria, Proteus mirabilis, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, Staphylococcus aureus, was cultivated for 33 tubes of broth culture. Leaving three tubes each group as control group, ploidy diluted concentration of high relative molecular weight chitosan, low relative molecular weight chitosan and sodium hyaluronate were added respectively in the broth culture. All the tubes were cultivated for 18 hours at 37 degrees C with homeothermia. Then the growth of bacteria was observed.
Results:
The minimal inhibitory concentrations (MIC) of high relative molecular weight chitosan were: Proteus mirabilis 0.031%, Escherichia coli 0.063%, Candida albicans 0.063%, Pseudomonas aeruginosa 0.063%, Staphylococcus aureus 0.063%; and the MIC of low relative molecular weight chitosan were: Proteus mirabilis 0.125%, Escherichia coli 0.025%, Candida albicans 0.25%, Pseudomonas aeruginosa 0.25%, Staphylococcus aureus 0.125%; bacteria grew well in each tube of sodium hyaluronate group and control group.
Conclusion:
The above results show that sodium hyaluronate has no bacteriostasis, while chitosan has bacteriostasis on broad spectrum and high relative molecular weigh: chitosan has stronger effect.