Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Sodium-iodide cotransporter in gene therapy].

B Czarnocka1

  • 1Zakładu Biochemii, Centrum Medycznego Kształcenia Podyplomowego i Zakładu Endokrynologii Instytutu, Centrum Medycyny Doświadczalnej i Klinicznej PAN w Warszawie. barbarac@cmkp.edu.pl

Wiadomosci Lekarskie (Warsaw, Poland : 1960)
|August 17, 2002
PubMed
Summary

Gene therapy using the sodium iodide symporter (NaIS) gene enables non-thyroidal cancer cells to accumulate radioiodine. This approach shows promise for targeted radiotherapy in various cancers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of endocrine disruptors on the reproductive system - current knowledge.

European review for medical and pharmacological sciences·2021
Same author

Effect of lithium carbonate on the function of the thyroid gland: mechanism of action and clinical implications.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2020
Same author

Procalcitonin in early onset ventilator-associated pneumonia.

The Journal of hospital infection·2012
Same author

Identification of chosen apoptotic (TIAR and TIA-1) markers expression in thyroid tissues from adolescents with immune and non-immune thyroid diseases.

Folia histochemica et cytobiologica·2010
Same author

Expression of Bcl-2 family proteins in thyrocytes from young patients with immune and nonimmune thyroid diseases.

Hormone research·2008
Same author

Identification of apoptotic proteins in thyroid gland from patients with Graves' disease and Hashimoto's thyroiditis.

Autoimmunity·2008

Area of Science:

  • Oncology
  • Molecular Biology
  • Radiotherapy

Background:

  • The sodium iodide symporter (NaIS) facilitates iodide uptake in thyroid cells.
  • Investigating NaIS gene transfer for novel cancer therapies is ongoing.
  • NaIS gene cloning and characterization occurred in 1996.

Purpose of the Study:

  • To explore the potential of NaIS gene transfer for cytoreductive gene therapy in non-thyroidal cancers.
  • To assess the efficacy of NaIS gene transfer in inducing radioiodine accumulation in cancer cells.

Main Methods:

  • Gene transfer of the NaIS gene into various non-thyroidal cancer cell lines.
  • In vitro and in vivo evaluation of radioiodine accumulation in transfected cells.
  • Demonstration of successful transfection in human ovarian adenocarcinoma, prostatic adenocarcinoma, glioma, melanoma, colon carcinoma, lung, and mammary gland cell lines.

Main Results:

  • NaIS gene transfer successfully induced radioiodine accumulation in multiple non-thyroidal cancer cell lines.
  • Transfected tumor cells demonstrated sufficient radioiodine uptake to elicit a therapeutic response to 131I.
  • Therapeutic efficacy was confirmed both in vitro and in vivo.

Conclusions:

  • NaIS gene transfer is a viable strategy for targeting radiotherapy in non-thyroidal cancers.
  • The NaIS gene holds potential as a novel therapeutic agent for cancer treatment.
  • This approach offers a promising avenue for targeted radioiodine therapy in diverse malignancies.

Related Experiment Videos