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A comparative study of Y chromosome microdeletions in infertile males from two Chinese populations
J Y M Tse1, W S B Yeung, E H Y Ng
1Department of Obstetrics and Gynaecology, The University of Hong Kong, Hong Kong, People's Republic of China. jymtse@hkucc.hku.hk
Purpose:
To compare the prevalence and type of Y-microdeletions in Hong Kong and Shanghai men with severe male-factor infertility.
Methods:
Seven Y-linked sequence tagged site (STS) primers and seven gene-specific primers were screened in 293 infertile males (139 from Hong Kong and 154 from Shanghai) and 161 fertile men (61 from Hong Kong and 100 from Shanghai). Serum FSH, LH, and testosterone levels were also measured in these men.
Results:
The incidence of Yq microdeletions in nonobstructive azoospermic men from Hong Kong (8.5%) and Shanghai (6%) was similar. Yq microdeletions were observed in severe oligospermic patients (8.5%) from Hong Kong but not from Shanghai. Among the 9 Hong Kong men with Y-microdeletions, 8 had AZFc deletion and one had AZFb deletion. In contrast, 6 of 9 men from Shanghai with Y-microdeletions had AZFb deletion. The incidence of AZFb deletion among Y-microdeleted men was statistically different between the two populations. Two of the men with AZFb deletion also had AZFa and AZFc deletions.
Conclusions:
Regional variations in the type of Y-microdeletion existed between Hong Kong and Shanghai infertile males.