Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

MacroH2A1.2 binds the nuclear protein Spop.

Ichiro Takahashi1, Yosuke Kameoka, Katsuyuki Hashimoto

  • 1Division of Genetic Resources, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan. itakahas@nih.go.jp

Biochimica Et Biophysica Acta
|August 17, 2002
PubMed
Summary

This study reveals that the macroH2A1.2 protein interacts with the Spop protein during X-chromosome inactivation. This interaction involves specific binding domains, shedding light on epigenetic regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of osteoblast cell-surface antigen regulating calcification as insulin receptor-related receptor, the alkali-sensor: A novel modulator of bone metabolism.

Histology and histopathology·2026
Same author

Inhibitor of DNA binding/differentiation 4 deficiency impairs hepatic fatty acid synthesis and is associated with epigenomic alterations in chromatin accessibility.

Molecular metabolism·2026
Same author

Wnt11 negatively regulates early chondrogenesis in a cell-type-dependent manner in embryonic mouse mesenchymal cells.

Biochemical and biophysical research communications·2026
Same author

Dietary pyrroloquinoline quinone and spermidine in healthy longevity: targeting the hallmarks of aging.

Frontiers in aging·2026
Same author

Vasculitis-associated aberrant apolipoprotein A2 = VAP2 and SDF-1α in Kawasaki disease patients with coronary artery lesion.

Microvascular research·2026
Same author

Fibroblast growth factor 8 regulates early Meckel's cartilage development via ERK signaling in a stage- and dose-dependent manner.

Developmental biology·2026

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Genetics

Background:

  • X-chromosome inactivation silences one X chromosome in female mammals.
  • Inactive X chromosomes are coated with Xist RNA and macroH2A1.2.
  • The function of macroH2A1.2's C-terminal region, including its leucine zipper domain, is unknown.

Purpose of the Study:

  • To investigate protein-protein interactions involving the nonhistone region of mouse macroH2A1.2.
  • To identify proteins that interact with macroH2A1.2.

Main Methods:

  • Yeast two-hybrid system for protein-protein interaction screening.
  • Glutathione-S-transferase (GST) pull-down assays for binding domain mapping.

Main Results:

Related Experiment Videos

  • MacroH2A1.2 was found to interact with mouse nuclear speckled type protein Spop.
  • Binding domain mapping indicated that macroH2A1.2 binds to Spop's MATH domain.
  • Spop's MATH domain binds to macroH2A1.2's putative leucine zipper domain.

    • Conclusions:

    • MacroH2A1.2 and Spop interact via specific domains.
    • This interaction may play a role in the function of macroH2A1.2 in X-chromosome inactivation.