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The 14-3-3s.

Robert J Ferl1, Michael S Manak, Matthew F Reyes

  • 1Program in Plant Molecular and Cellular Biology, Department of Horticultural Sciences, University of Florida, Gainesville, FL 32611, USA. robferl@ufl.edu

Genome Biology
|August 20, 2002
PubMed
Summary

14-3-3 proteins are essential eukaryotic adapters regulating stress response, cell cycle, and apoptosis. Phylogenetic analysis reveals distinct plant 14-3-3 groups, highlighting conserved and divergent regions involved in protein interactions.

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Area of Science:

  • Molecular Biology
  • Protein Biochemistry
  • Evolutionary Biology

Background:

  • 14-3-3 proteins are conserved across eukaryotes, absent in prokaryotes.
  • They function as homo- and hetero-dimers, interacting with client proteins.
  • These interactions regulate diverse cellular processes like stress response, cell cycle control, and apoptosis.

Purpose of the Study:

  • To analyze the phylogenetic relationships within the 14-3-3 protein family.
  • To investigate the structural basis for client protein binding and localization.

Main Methods:

  • Phylogenetic analysis of 133 full-length 14-3-3 protein sequences.
  • Examination of conserved and divergent regions within the protein sequences.

Main Results:

  • Phylogenetic analysis identified two distinct groups within the plant 14-3-3 family.
  • The conserved core region contains an amphipathic groove for client protein binding.
  • Divergent amino and carboxyl termini suggest isoform-specific interactions and localization.

Conclusions:

  • The 14-3-3 protein family exhibits conserved and divergent regions crucial for its diverse functions.
  • Structural variations contribute to specialized client interactions and subcellular localization.
  • Phylogenetic insights aid in understanding the evolutionary and functional diversification of 14-3-3 proteins.

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