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Related Experiment Videos

Functional genomics in the mouse.

Archibald S Perkins1

  • 1Pathology and Molecular, Cellular, Developmental Biology, Yale University, P.O. Box 208023, New Haven, CT 06520-8023, USA. archibald.perkins@yale.edu

Functional & Integrative Genomics
|August 20, 2002
PubMed
Summary
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Mice are crucial genetic models for studying human disease. This review explores mutagenesis tools like ethylnitrosourea and cre-lox systems for uncovering mouse gene functions.

Area of Science:

  • Genetics and Genomics
  • Developmental Biology
  • Disease Modeling

Background:

  • The mouse is a primary genetic model organism for human disease and development studies.
  • Advances in genome sequencing have spurred interest in large-scale gene function analysis.
  • Understanding gene function is critical for biological and medical research.

Purpose of the Study:

  • To review available tools for mouse genome manipulation and mutagenesis.
  • To discuss how these tools are applied to uncover gene function.
  • To highlight the importance of mutagenesis technologies in genetic research.

Main Methods:

  • Ethylnitrosourea (ENU) mutagenesis
  • Gene trap mutagenesis
  • Cre-lox system for engineered inversions and deletions

Related Experiment Videos

  • Proviral insertional mutagenesis in somatic cells

    • Main Results:

    • These methods provide powerful approaches for large-scale genetic screens.
    • Mutagenesis technologies enable systematic disruption and analysis of gene function.
    • The cre-lox system allows for targeted genomic modifications.

    Conclusions:

    • A variety of effective tools exist for mouse genome manipulation.
    • These tools are essential for deciphering gene function in the context of disease and development.
    • Continued development and application of these technologies will advance biological understanding.