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TIP49, but not TRRAP, modulates c-Myc and E2F1 dependent apoptosis
Kerri A Dugan1, Marcelo A Wood, Michael D Cole
1Department of Molecular Biology, Princeton University, New Jersey 08544-1014, USA.
Oncogene
|August 20, 2002
Summary
Nuclear cofactors TRRAP and TIP49 are crucial for oncogenesis. While both are vital for cell transformation, TIP49 uniquely influences apoptosis, impacting both c-Myc and E2F1 functions.
Area of Science:
- Molecular Biology
- Cancer Research
- Cell Biology
Background:
- Two nuclear cofactors, TRRAP and TIP49, were previously identified for their roles in Myc-mediated oncogenesis.
- Further investigation into these Myc-associated cofactors is needed to understand their broader functions.
Purpose of the Study:
- To investigate the roles of TRRAP and TIP49 in apoptosis and cell proliferation.
- To determine how these cofactors modulate the functions of c-Myc and E2F1.
Main Methods:
- Analysis of TRRAP and TIP49 function in cellular processes.
- Assessment of cofactor roles in apoptosis and cell proliferation.
- Investigation of cofactor binding and modulation of transcription factors c-Myc and E2F1.
Main Results:
- Both TRRAP and TIP49 are essential for cellular transformation.
- TIP49 modulates c-Myc-mediated apoptosis, while TRRAP disruption shows no significant effect on apoptosis.
- TIP49 also binds to the E2F1 transactivation domain, modulating its transforming and apoptotic activities.
Conclusions:
- Individual nuclear cofactors differentially regulate the functions of c-Myc and E2F1.
- TIP49 plays a distinct role in modulating apoptosis in response to c-Myc and E2F1 signaling.