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Updated: Sep 29, 2026

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
Protein denitration/modification by glutathione-S-transferase and glutathione peroxidase
Wu-Nan Kuo1, Joseph M Kocis, Madhvi Mewar
1Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, FL 32114, USA. kuow@cookman.edu
Abstract:
Peroxynitrite (PN)-pretreated histone III-S (NH) and reduced glutathione (GSH)-treated NH (NH(GSH)) were incubated with glutathione-S-transferase (GST) and glutathione peroxidase (GPX). Western blot analysis revealed decreased 3-nitrotyrosine immunoreactivity for NH(GSH), but not for NH. Additionally, increased nitrate was noted as an end product of these enzymatic reactions. The findings imply that GSH-treatment of NH may facilitate its conformational change in favor of subsequent enzymatic denitration and/or modification, which could be vital in relieving cellular oxidative stress and regulating NO/PN-mediated signal transduction cascade.
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