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Chromosomal aberrations in transitional cell carcinoma: its correlation with tumor behavior
Dah-Shyong Yu1, Hong-I Chen, Sun-Yran Chang
1Uro-Oncology Laboratory, Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, National Defense College, Taipei, Taiwan/ROC. yuds@ms21.hinet.net
Urologia Internationalis
|August 21, 2002
Summary
This study used fluorescence in situ hybridization (FISH) to analyze chromosome number changes in transitional cell carcinoma (TCC) of the urinary tract. Chromosomes 11 and 15 stability correlated with tumor grade and stage, unlike other chromosomes.
Area of Science:
- Cytogenetics
- Uro-oncology
- Molecular diagnostics
Background:
- Transitional cell carcinoma (TCC) is the most common type of bladder cancer.
- Understanding chromosomal aberrations in TCC is crucial for diagnosis and prognosis.
Purpose of the Study:
- To investigate numerical chromosomal aberrations in TCC cell lines using fluorescence in situ hybridization (FISH).
- To correlate chromosomal abnormalities with DNA ploidy, tumor grade, and stage.
Main Methods:
- FISH analysis of chromosomes 1, 3, 7, 9, 11, 15, 17, X, and Y in 16 TCC cell lines.
- Analysis of at least 200 nuclei per cell line.
- DNA ploidy assessment via propidium iodide flow cytometry.
Main Results:
- Aneuploidy was common in TCC cell lines, with significant polysomy for chromosomes 1, 7, 9, 17, and X.
- Chromosome Y loss occurred in 83% of male-derived TCC cell lines.
- Disomy of chromosome 11 correlated with lower grade TCC, while disomy of chromosome 15 correlated with higher grade TCC and higher tumor stage.
Conclusions:
- Polysomy of chromosomes 1, 7, 9, 17, and X is prevalent in aneuploid TCC.
- Chromosomes 11 and 15 exhibit stability linked to TCC tumor grade and stage.
- The significance of chromosome Y loss and chromosome 3 monosomy in bladder cancer remains undetermined.