Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diabetic cystopathy correlates with a long-term decrease in nerve growth factor levels in the bladder and lumbosacral

Katsumi Sasaki1, Michael B Chancellor, Michael W Phelan

  • 1Departments of Urology and Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.

The Journal of Urology
|August 21, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Solvent-Guided Galvanic Replacement of Ag on Cu Nanowires: Dynamic Structural Evolution for CO<sub>2</sub> Electrolysis.

Chemistry, an Asian journal·2026
Same author

Outcome and Impact of Diagnostic Ureteroscopy in Clinically Suspected Upper Urinary Tract Carcinoma <i>in Situ</i>.

Journal of endourology·2025
Same author

Dissecting Superficial Inferior Epigastric Veins Using a Tablet Navigation Device.

Plastic and reconstructive surgery. Global open·2025
Same author

Polyploid superficial uroepithelial bladder barrier cells express features of cellular senescence across the lifespan and are insensitive to senolytics.

Aging cell·2024
Same author

Prognostic impact of radiological tumor burden in patients with metastatic urothelial carcinoma treated with pembrolizumab.

Urologic oncology·2023
Same author

Redox Activity of Ir<sup>III</sup> Complexes with Multidentate Ligands Based on Dipyrido-Annulated N-Heterocyclic Carbenes: Access to High Valent and High Spin State with Carbon Donors.

Chemistry (Weinheim an der Bergstrasse, Germany)·2023

Diabetic rats showed decreased nerve growth factor (NGF) levels, leading to bladder dysfunction. Reduced NGF in the bladder and dorsal root ganglia contributes to diabetic cystopathy.

Area of Science:

  • Urology
  • Neuroscience
  • Endocrinology

Background:

  • Diabetic neuropathy is linked to impaired axonal transport of nerve growth factor (NGF).
  • This impairment may contribute to diabetic cystopathy, a bladder dysfunction in diabetes.
  • Understanding the role of NGF in diabetic bladder function is crucial for treatment development.

Purpose of the Study:

  • To investigate the relationship between bladder function and NGF levels in diabetic rats.
  • To examine NGF levels in the bladder and dorsal root ganglia (DRG) in streptozotocin-induced diabetes.
  • To assess the impact of diabetes on bladder afferent nerve function (Aδ and C-fibers).

Main Methods:

  • Diabetes was induced in rats using streptozotocin.
  • Bladder function was assessed using metabolic cage measurements and awake cystometry.

Related Experiment Videos

  • NGF levels were measured using ELISA in bladder and L6-S1 DRG at various time points.
  • Bladder nociceptive responses were evaluated using cystometry with acetic acid instillation.

    • Main Results:

    • Diabetic rats exhibited significantly decreased NGF levels in the bladder and DRG at 12 weeks post-induction.
    • Bladder capacity increased, and post-void residual volume significantly rose in diabetic rats.
    • Time-dependent reduction in bladder nociceptive responses was observed in diabetic rats.

    Conclusions:

    • Streptozotocin-induced diabetes leads to a time-dependent decrease in NGF levels in the bladder and DRG.
    • This reduction in NGF is associated with voiding dysfunction due to impaired Aδ and C-fiber bladder afferents.
    • Reduced NGF production or impaired transport may be a key mechanism underlying diabetic cystopathy.