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[Evaluation of liver function with 13C-labelled amino acid using hepatectomized rat model]
Yukimoto Ishii1, Koichi Ishikawa, Satoshi Asai
1Department of Surgery Division 3, Nihon University School of Medicine, Itabashi-ku, Tokyo 173-8610, Japan. yishii@med.nihon-u.ac.jp
Unlabelled:
Using a rat model of hepatectomy, we investigated whether the severity of hepatopathy could be quantitatively measured from changes in expiratory 13CO2 levels after intravenous administration of L-[1-(13)C]phenylalanine, L-[1-(13)C]methionine or L-[1-(13)C]alanine.
Materials And Methods:
Under nembutal anesthesia, 30 mg/kg L-[1-(13)C]phenylalanine, 40 mg/kg L-[1-(13)C]methionine or 20 mg/kg L-[1-(13)C]alanine was administered to rats through the femoral vein, and expiratory 13CO2 levels were measured for 15 min. Thirty percent, 70% or 90% hepatectomy was performed. In the control group, simple laparotomy was performed.
Results:
The correlation coefficient between total 13CO2 output over 15 min after L-[1-(13)C]phenylalanine administration and liver weight/body weight was 0.883 (P < 0.001). The correlation coefficient between total 13CO2 output over 15 min after L-[1-(13)C]methionine administration and liver weight/body weight was 0.922 (P < 0.001). The correlation coefficient between total 13CO2 output over 15 min after L-[1-(13)C]alanine administration and liver weight/body weight was 0.902 (P < 0.0001).
Conclusion:
In the breath test with intravenously administered L-[1-(13)C]phenylalanine, L-[1-(13)C]methionine, or L-[1-(13)C]alanine, hepatopathy could be quantitatively evaluated by measuring expiratory 13CO2 levels over 15 min.