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Updated: Sep 29, 2026

Alternative Methods for the Detection of Superoxide Anion Generation in Platelets
Published on: March 29, 2024
Peroxynitrite-mediated modification of fibrinogen affects platelet aggregation and adhesion
Pawel Nowak1, Barbara Wachowicz
1Department of General Biochemistry, University of Lodz, Lodz, Poland. pnowak@biol.lodz.pl
Abstract:
The reaction of peroxynitrite with fibrinogen resulted in both structural modifications and altered biological properties of this glycoprotein. SDS-PAGE analysis of peroxynitrite-treated fibrinogen, performed under non-reducing conditions, showed some aggregated material on the top of the gel (5-10% of total staining bands) and the presence of nitrotyrosine. The amount of nitrotyrosine, detected by immunoassay with anti-nitrotyrosine antibodies, was dependent on peroxynitrite concentration. In comparison with native molecule, peroxynitrite-treated fibrinogen subjected to SDS-PAGE under reducing conditions revealed not only three bands corresponding to Aalpha, Bbeta and gamma chains, but the existence of additional high molecular bands probably due to the formation of dityrosine crosslinking between fibrinogen subunits. The different susceptibility in tyrosine nitration of fibrinogen subunits was also observed. The Aalpha chain was the most intensely nitrated, while Bbeta and gamma chains were nitrated much less? Peroxynitrite-treated fibrinogen in comparison with native molecule had a distinct capability to mediate platelet adhesion and aggregation. Both unstimulated and ADP-activated platelets showed a reduced ability to adhere to peroxynitrite-modified fibrinogen. The percentage of ADP-induced platelet aggregation decreased as a function of peroxynitrite-mediated modification of fibrinogen molecule.
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