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Large multifunctional proteasome 2 gene Arg60His variant in autoimmune thyroid disease in Shanghai Chinese
Hong Ding1, Yi Shi, Kunsan Xiang
1Department of Endocrinology and Metabolism, The Sixth People Hospital Shanghai 200233, China
Objective:
To investigate the relationship between Arg60His variant in the large multifunctional proteasome 2(LMP2) gene and autoimmunune thyroid disease (AITD) in Shanghai Chinese.
Methods:
By means of PCR-restriction fragment length polymorphisms(RFLP) assay, the genotypes of Arg60His variant in the LMP2 gene were determined in 367 unrelated Shanghai Chinese, including 205 patients with AITD and 162 normal control individuals. The former group was further divided into two subgroups 78 subjects with positive thyrotrophin receptor antibody(TRAb) and 127 subjects with negative TRAb but positive either thyroglobulin antibody(TGAb),thyoid microsomal antibody(TMAb) or thyroid peroxidase antibody (TPOAb).
Results:
Significant difference ( P = 0.000)of allele frequencies of R/H 60 in the LMP2 gene was observed between Shanghai Chinese (R 0.89,H 0.11) and white Caucasians (R 0.29, H 0.71)and was also observed between TRAb positive subgroup in AITD and normal controls. The frequencies of RH genotype and H allele in TRAb positive subgroup is significantly higher than those in normal controls( P = 0.037,0.047,respectively).In AITD group, the genotype and allele frequencies of Arg60His variant in the LMP2 gene showed no significant difference between other antibody(+) subgroups and antibody(-) subgroups.
Conclusion:
Arg60His variant in the LMP2 gene may be associated with the pathogenesis of AITD in Shanghai Chinese.