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A RP-LC method with evaporative light scattering detection for the assay of simethicone in pharmaceutical
Douglas E Moore1, Tina X Liu, William G Miao
1Faculty of Pharmacy, The University of Sydney, Sydney 2006, Australia. demoore@pharm.usyd.edu.au
Abstract:
A reversed-phase liquid chromatographic method has been developed and validated for the determination of the polydimethylsiloxane (PDMS) component of Simethicone, which is used as an anti-foaming agent in pharmaceutical formulations. The method involves acidification to neutralise antacid components of the formulation, then a single extraction of the PDMS with dichloromethane. This is followed by separation with a reversed-phase column using an acetonitrile-chloroform solvent gradient, and quantification by an evaporative light scattering detector. An assay precision of 3% was achieved in intraday and interday determinations. No interference was found from the aluminium and magnesium hydroxide components of antacid formulations.
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