Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A comparison of imaging methods for use in an array biosensor.

Joel P Golden1, Frances S Ligler

  • 1Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, Code 6900, Washington, DC 20375-5348, USA. jgolden@cbmse.nrl.navy.mil

Biosensors & Bioelectronics
|August 23, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optical Analysis of Cyclic Voltammetry of Ferrocenemethanol: A Comparative Study of SPR and LSPR.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Smart Catheters for Diagnosis, Monitoring, and Therapy.

Advanced healthcare materials·2025
Same author

Advances in biosensors for diagnosis of Alzheimer's and Parkinson's diseases.

Biosensors & bioelectronics·2025
Same author

Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells.

Nature biotechnology·2025
Same author

Overcoming the effects of fluid shear stress in ovarian cancer cell lines: Doxorubicin alone or photodynamic priming to target platinum resistance.

Photochemistry and photobiology·2024
Same author

Forty years of advances in optical biosensors-are "autonomous" biosensors in our future?

Analytical and bioanalytical chemistry·2024
Same journal

Recent developments of textile-based triboelectric nanogenerators for smart sports applications.

Biosensors & bioelectronics·2026
Same journal

One-Tube RPA-CRISPR-Cas13a assay with rational design for single-molecule detection of waterborne viruses in drinking water treatment.

Biosensors & bioelectronics·2026
Same journal

AI-driven photophysics-aware design of fluorescent probes with applications in α-synuclein biosensing and inhibitor screening.

Biosensors & bioelectronics·2026
Same journal

Three-dimensional helical integration of high-density linear microelectrode arrays and their cross-tissue applications.

Biosensors & bioelectronics·2026
Same journal

Integration of electrochemical sensors in organ-on-a-chip microfluidic platforms: Advances and perspectives.

Biosensors & bioelectronics·2026
Same journal

DNN-PURE: A deep neural network approach to paper-based urea sensing.

Biosensors & bioelectronics·2026
See all related articles

This study compared a cooled charge-coupled device (CCD) imager with a complementary metal-oxide semiconductor (CMOS) camera and photodiode for array biosensor applications. The CMOS camera and photodiode showed comparable signal discrimination to the CCD, offering potential cost and space savings.

Area of Science:

  • Biomedical Engineering
  • Optical Sensing Technologies
  • Biosensor Development

Background:

  • Array biosensors commonly utilize actively-cooled charge-coupled device (CCD) imagers for sensitive detection.
  • Cooling systems in CCD imagers add significant cost, size, and power requirements.
  • Exploring alternative, more economical, and compact detection systems is crucial for advancing biosensor technology.

Purpose of the Study:

  • To evaluate complementary metal-oxide semiconductor (CMOS) cameras and photodiodes as cost-effective replacements for cooled CCD imagers in array biosensors.
  • To compare the signal discrimination capabilities of CMOS and photodiode detectors against a standard cooled CCD imager.
  • To assess the feasibility of using simpler detection systems without compromising performance.

Main Methods:

Keywords:
NASA Discipline Life Sciences TechnologiesNon-NASA Center

Related Experiment Videos

  • An array biosensor setup was employed with identical imaging optics for all tests.
  • Different concentrations of CY5 fluorescent dye in glycerol were used as the imaging target.
  • Signal-to-noise ratios were measured and compared across three detector types: cooled CCD, CMOS camera, and photodiode.

Main Results:

  • The complementary metal-oxide semiconductor (CMOS) camera demonstrated signal discrimination comparable to the actively-cooled charge-coupled device (CCD) imager.
  • The photodiode also exhibited viable signal discrimination, though potentially with different performance characteristics.
  • Both CMOS and photodiode systems offered potential for reduced cost and spatial footprint compared to the cooled CCD.

Conclusions:

  • Complementary metal-oxide semiconductor (CMOS) cameras present a viable, lower-cost, and more compact alternative to cooled charge-coupled device (CCD) imagers for array biosensor applications.
  • Photodiodes may also serve as a suitable detection component, warranting further investigation for specific applications.
  • These findings support the development of more accessible and portable biosensing platforms.