Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Large-scale mutagenesis and functional genomics in yeast.

Qiang Q Que1, Elizabeth A Winzeler

  • 1Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121, USA.

Functional & Integrative Genomics
|August 23, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasmodium falciparum HSP90 inhibitors show divergent resistance despite a shared ATP-binding site.

Cell reports·2026
Same author

Survival differences and artemisinin resistance in severe malaria among HIV coinfected patients: data from Mozambique.

medRxiv : the preprint server for health sciences·2026
Same author

AI-Accelerated Structure Elucidation of Boavistamides A-C, Cyclic Depsipeptides from a Marine Filamentous Cyanobacterium Collected in Cabo Verde.

bioRxiv : the preprint server for biology·2026
Same author

AI-Accelerated Structure Elucidation of Boavistamides A<b>-</b>C, Cyclic Depsipeptides from a Marine Filamentous Cyanobacterium Collected in Cabo Verde.

Journal of natural products·2026
Same author

Duplication of superoxide dismutase and a mutation in aquaglyceroporin mediates the sensitivity of <i>Plasmodium falciparum</i> to cryptosporin, a natural product derived from <i>Acaromyces ingoldii</i>.

bioRxiv : the preprint server for biology·2026
Same author

Structure and functional diversity of antibodies targeting the <i>P. falciparum</i> circumsporozoite protein C-terminal domain.

bioRxiv : the preprint server for biology·2026

Researchers are systematically deleting every gene in yeast to understand protein functions. This genome-wide mutagenesis approach in Saccharomyces cerevisiae helps determine gene roles by analyzing mutant phenotypes.

Area of Science:

  • Molecular Biology
  • Genetics
  • Yeast Biology

Background:

  • Determining protein function is a key post-genome sequencing challenge.
  • Traditional methods like comparative sequencing and proteomics are often indirect.
  • Saccharomyces cerevisiae offers a unique model for direct gene function analysis.

Purpose of the Study:

  • To describe progress in yeast genome-wide mutagenesis programs.
  • To report findings from analyzing systematically created gene deletion mutants.

Main Methods:

  • Systematic gene deletion in Saccharomyces cerevisiae.
  • Phenotypic analysis of generated mutants.
  • Genome-wide mutagenesis strategies.

Main Results:

Related Experiment Videos

  • Progress in yeast genome-wide mutagenesis programs detailed.
  • Analysis of mutants created through systematic gene deletion.
  • Insights into gene function derived from mutant phenotypes.

Conclusions:

  • Systematic gene deletion in yeast is a powerful tool for functional genomics.
  • This approach facilitates direct testing of gene roles and phenotype analysis.
  • Genome-wide mutagenesis in Saccharomyces cerevisiae advances understanding of protein function.