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Related Experiment Videos

Sema7A is a potent monocyte stimulator.

S Holmes1, A M Downs, A Fosberry

  • 1Oxford GlycoSciences, Adbingdon, Oxon, UK. steve.holmes@ogs.co.uk

Scandinavian Journal of Immunology
|August 24, 2002
PubMed
Summary

Semaphorin 7A (Sema7A) potently activates monocytes, stimulating inflammatory responses and promoting dendritic cell morphology. It acts as an autocrine activator for monocytes, influencing immune cell behavior.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Semaphorin 7A (Sema7A) is a cell surface protein linked via a glycophosphatidylinositol anchor.
  • Its biological roles, particularly in immune cell activation, require further elucidation.

Purpose of the Study:

  • To investigate the mRNA expression and biological functions of Semaphorin 7A (Sema7A).
  • To determine the specific effects of Sema7A on various immune cell types, including monocytes, lymphocytes, and neutrophils.

Main Methods:

  • Analysis of Sema7A mRNA expression in different cell types.
  • Functional assays to assess Sema7A's impact on cytokine production, proliferation, chemotaxis, and cell morphology.

Main Results:

  • Sema7A mRNA is expressed in lymphoid and myeloid cells.

Related Experiment Videos

  • Sema7A demonstrated potent activation of monocytes, inducing chemotaxis, inflammatory cytokine release (IL-1β, TNF-α, IL-6, IL-8), and superoxide production.
  • Sema7A also stimulated granulocyte-macrophage colony-stimulating factor (GM-CSF) production and induced a dendritic cell morphology in monocytes.
  • Sema7A showed less efficacy in activating neutrophils and no significant effect on B or T cell proliferation or cytokine production.

    • Conclusions:

    • Sema7A is a potent activator of monocytes, acting through an autocrine mechanism.
    • Sema7A plays a significant role in modulating monocyte function and differentiation, contributing to inflammatory processes.