Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

BRCA2 monoclonal antibodies react with differentiating epithelium.

Lisa K Gilliam1, Edward K Lobenhofer, Paula K Greer

  • 1Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA.

Hybridoma and Hybridomics
|August 24, 2002
PubMed
Summary

New immunological reagents reveal the BRCA2 gene product is involved in cellular differentiation. This finding expands on its known roles in DNA repair and proliferation, offering insights into hereditary breast cancer.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptomic subtypes in high-grade serous ovarian cancer are driven by tumor cellular composition.

bioRxiv : the preprint server for biology·2026
Same author

Identification of cycling regulatory T cell precursors as conductors of immune escape during breast carcinoma progression.

Cancer cell·2026
Same author

Aggressive Neuroblastomas Start Growing after Infancy.

bioRxiv : the preprint server for biology·2026
Same author

Medication adherence and persistence of newly initiated medications in older adults with diabetes.

Journal of the American Pharmacists Association : JAPhA·2026
Same author

Spatial proteomics of breast ductal carcinoma in situ reveal distinct regional differences.

Scientific reports·2026
Same author

Prognostic Implications of Codon-Specific <i>KRAS</i> Mutations in Localized and Advanced Stages of Pancreatic Cancer.

JCO precision oncology·2026

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • The BRCA2 gene is implicated in DNA repair and cell proliferation.
  • Germline mutations in BRCA2 are linked to hereditary breast cancer.
  • Understanding BRCA2 expression and function is crucial.

Purpose of the Study:

  • To develop specific immunological reagents for BRCA2 detection.
  • To investigate the expression pattern and function of the BRCA2 gene product.
  • To explore BRCA2's role beyond DNA repair and proliferation.

Main Methods:

  • Development of immunological reagents specific for BRCA2.
  • Testing reagent reactivity with recombinant BRCA2 proteins.
  • Detection of native BRCA2 protein using immunoprecipitation-Western blot analysis.

Related Experiment Videos

  • Assessing reagent reactivity in various differentiating epithelial tissues.

    • Main Results:

    • Developed reagents recognize full-length and smaller BRCA2 polypeptides.
    • Sensitive detection methods are required for most tissue types.
    • Demonstrated strong reactivity with differentiating epithelium (epidermis, thymic epithelium, squamous cell carcinoma).

    Conclusions:

    • BRCA2 protein is detectable in differentiating epithelial tissues.
    • BRCA2 likely plays a role in cellular differentiation.
    • These findings complement known roles in proliferation and DNA repair, impacting hereditary cancer research.